badionotus, and on most other species of water cucumbers, look throughout these exploited variety for the Colombia has been limited by several degree into the variety and shipment [2, 16] and you will reproductive biology [17, 18]. However, you may still find of a lot openings into the knowledge, such regarding their reproductive models as well as how they are determined by parameters particularly heat, salinity, rainfall, and you will dinner supply.
Today’s studies relates to regions of the newest reproductive biology of the holothuroids We. badionotus and you will Isostichopus sp. in the Santa Marta Area making use of the gonadal directory (GI) and you can histological study of the new gonads. This study is meant to improve newest understanding of the fresh biology and you will ecology of ocean cucumbers indigenous to the brand new Colombian Caribbean Sea and gives a technical cause for the development of management methods and you can conservation in the funding.
dos. Material and methods
Specimens out of Isostichopus sp. was indeed compiled regarding , if you’re specimens out-of Isostichopus badionotus were collected away from from the Taganga Bay (11° 16′ ” N– 74° 11′ ” W) and you may Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) on Caribbean linked here area for Santa Marta, Colombia (Fig step one).
In addition it evaluates the fresh dictate regarding environmental and you will agents variables (temperature, rainfall, the fresh portion of normal carbon dioxide posts regarding sediment) on the reproductive biology and you may analyzes the structure of their communities (frequency of size and you will weight, gender ratio, imply dimensions and you can weight to start with intimate readiness, fecundity)
Package from inside the inset A is actually magnified during the inset B; package during the inset B try magnified however panel. Source: Information feet INVEMAR.
Considering the wide variety of molds and colors which i. badionotus has, this study focused on the new line of organisms with the most common morphotype in the variety within the analysis city, demonstrated by Vergara mais aussi al. whenever i. badionotus morphotype II.
Month-to-month fourteen to 20 individuals of each morphotype (We. badionotus and you can Isostichopus sp.) was basically built-up because of the snorkeling. For this reason, a pair of snorkelers swam an effective transect 5 meters wider because of the whenever one thousand m much time, double. The initial travel was made bordering the new coastline range additionally the next far away of around 10 m on the coastline range. Maximum lookup breadth try ten m for logistical and you can defense reasons and although it is likely that you will find anybody from the higher distance and you may depth, this could not be assessed in the modern study. In the October, zero selections was generated considering the passage through of Hurricane Matthew, and therefore impacted the fresh Colombian Caribbean throughout that few days. All the sea cucumbers was myself packed from inside the synthetic bags having seawater and you may transferred on the Aquaculture Laboratory off Universidad del Magdalena. The brand new breadth out of grab of every personal additionally the variety of bottom they filled was basically submitted (categorized since rocky, sandy, coral, rock-red coral, rock-sand, rock-sand-coral). Simultaneously, facial skin sediment samples (with a density not greater than ten mm) was in fact removed near the men and women to dictate the fresh new part of organic carbon articles playing with a bulk spectrometer, Euro EA 3000 essential analyzer (EuroVector). Salinity and you will liquids temperature was indeed submitted monthly, if you find yourself complete rain data have been obtained from the fresh new information of your own Simon Bolivar airport meteorological station of your town of Santa Marta.
The folks have been sacrificed in the research of the place him or her within the seawater on 4°C, which was and additionally meant to arrive at a max condition off contraction that permitted to reduce the mistake through the duration proportions, as a result of the skill that they must continue or contract within often. Then your following the body dimensions were pulled: overall body length (TL), playing with an adaptable step 1-meters tape scale within nearby 0.5 cm (once sacrifice of the thermal shock the fresh developed body got a-c otherwise S profile, plus the tape contoured you, therefore, the complete size ‘s the profile duration); gonad frequency playing with a graduated tube at nearest 0.step one cm step three ; body wall structure lbs (BW), recognized as the extra weight of the individual versus viscera otherwise gonad and gonad lbs (GW) at the nearest 0.1 g. Gonad list (GI) is actually calculated making use of the formula, GI = (GW/ BW)* one hundred [19, 20].